Figure 3
From: Intrinsic predisposition of naïve cystic fibrosis T cells to differentiate towards a Th17 phenotype
Naїve T cells from CF subjects are intrinsically predisposed to Th17 differentiation. (A) Relative mRNA expression levels of CFTR on naїve human CD4+ T cells following antibody stimulation at different time-points. (B) Representative flow cytometry plots of IFN-γ production by naїve T cells from healthy controls and CF subjects following Th1 differentiation. (C) Proportions of IFN-γ- producing Th1 cells. (D) Representative flow cytometry plots of Foxp3 expression by naїve T cells from healthy controls and CF subjects following differentiation into Tregs. (E) Proportions of Foxp3+ Tregs. (F) Representative flow cytometry plots of IL-17 production by naїve T cells from healthy controls and CF subjects following Th17 differentiation. (G) Proportions of IL-17- producing Th17 cells. 5 CF subjects and 3 healthy controls, representative of 3-4 independent experiments. Mean ± SEM. *p < 0.05.