Figure 1
From: Intrinsic predisposition of naïve cystic fibrosis T cells to differentiate towards a Th17 phenotype
CFTR-/- T cells are intrinsically predisposed to differentiate along the Th17 lineage. (A) Relative mRNA expression of CFTR in naїve CD4+ T cells from CFTR+/+ mice following stimulation with anti-CD3 and anti-CD28 antibodies at different time points. (B-J) Naїve CD4+ T cells from CFTR+/+ and CFTR-/- mice were differentiated into Th1, Treg and Th17 lineages respectively in the presence of inducing cytokines. (B) Flow cytometry plots of IFN-γ production by CD4+ T cells. (C) Proportions of IFN-γ producing cells generated under Th1 inducing conditions. (D) Levels of IFN-γ released by differentiated cells. (E) Flow cytometry plots of Foxp3 expression by CD4+ T cells. (F) Proportions of Foxp3+ cells generated under Treg inducing conditions. (G) Levels of TGF-β released by differentiated cells. (H) Flow cytometry plots of IL-17 production by differentiated cells. (I) Proportions of IL-17 producing cells generated under Th17 inducing conditions. (J) Levels of IL-17 released by differentiated cells. n = 4-6 mice per group, representative of 2-3 independent experiments. Mean ± SEM, * p < 0.05 vs CFTR +/+.