Figure 4
Tight junction formation of immortalized BCi-NS1.1 cells during air-liquid interface culture. A. Transepithelial electric resistance (TER) of BCi-NS1.1 cells cultured on air-liquid interface (ALI). Resistance (Ohms x cm2) was measured at every media change. Data shown is the average TER ± the standard error of n = 8 independent experiments from passage 6–30 cells between day 10 to 40 of ALI culture. B. Expression of tight junction-related genes of BCi-NS1.1 cells. At ALI day 0 and day 28, TaqMan quantitative PCR analysis was used to assess expression of the tight junction related genes claudin 3 (CLDN3) and claudin 8 (CLDN8). Data shown is the average ± the standard error of n = 10 independent experiments for BC-NS1.1 cells from passage 6 to 43. Statistics were calculated by a 2-tailed Student’s t test.