Table 2 Antibody staining panel
From: Lung CD8+ T cells in COPD have increased expression of bacterial TLRs
Fluorochrome | 1 | 2 | 3 | 4 | 5 | 6 |
|---|---|---|---|---|---|---|
FITC | M IgG2a | TLR5 | CD45 | CD45 | CD45 | CD45 |
PE | IC:M IgG1 | IC:TLR3 | M IgG1 | TLR1 | IC:R IgG2a | IC:TLR9 |
Biotin (PE-Cy5) | Â | Â | R IgG2a | TLR6 | Â | Â |
PE-Cy7 | CD45 | CD45 | M IgG1 | TLR2 | M IgG2a | TLR4 |
APC | M IgG2a | CD56 | M IgG2a | CD56 | M IgG2a | CD56 |
APC-Cy7 | M IgG1 | CD3 | M IgG1 | CD3 | M IgG1 | CD3 |
Pacific Blue | M IgG2a | CD8 | M IgG2a | CD8 | M IgG2a | CD8 |
Alexa Fluor 700 | M IgG2b | CD4 | M IgG2b | CD4 | M IgG2b | CD4 |
Aqua (AmCyan) | Live/Dead | Live/Dead | Live/Dead | Live/Dead | Live/Dead | Live/Dead |