Figure 2

Isolation of LysoTracker^posalveolar type II cells. FACS plots showing the sequential gating strategy used to isolate AT2 cells. AT2 cells were gated on the basis of A) size (FSC vs. SSC), B) viability (PI^neg) and the exclusion of C-D) cell doublets. Viable E) non-haematopoietic cells (CD45^neg) and F) non-endothelial cells (CD31^neg) were then gated on prior to sorting AT2 cells on the basis of their differential expression of G) EpCAM and Lysotracker staining. H) Histogram showing viability analysis (PI) of sorted EpCAM^pos LysoTracker^pos cells at 4 hours post-sort.