Figure 1

Pseudomonas aeruginosa (PA) infection reduces SPLUNC1 and increases neutrophil elastase (NE) activity in bronchoalveolar lavage (BAL) fluid of wild-type (WT) mice. BAL fluid from WT mice was processed for SPLUNC1 protein Western blot. (A) – Quantitative analysis of BAL fluid SPLUNC1 protein expression normalized by albumin. (B) – Representative Western blot image of SPLUNC1 and albumin. The vertical dotted red line separates the saline group from the PA infection group. The two lanes under saline or PA treatment represent SPLUNC1 data from two different mice. (C) – NE activity was examined by an NE activity assay as described in Materials and methods. N = 4 – 5 mice per group. Data are expressed as means ± SEM.