Figure 7
From: Nicotine exposure during differentiation causes inhibition of N-myc expression
N-myc is decreased after differentiation in the presence of nicotine. A. The decrease in N-myc expression caused by nicotine exposure during differentiation was detected by array and confirmed here by real-time PCR. In addition, this demonstrates that fibroblasts exposed to nicotine after differentiation have the opposite response. B. N-myc protein expression was quantified by immunoblotting at passages 8, 9, and 13. The top panel shows decreased N-myc protein after nicotine treatment as determined by LiCor imaging analysis, with fluorescence intensity for each sample normalized to β-actin. The bottom panel is a representative blot, showing protein expression at passage 8. Changes between untreated and nicotine treated cells are significant, P < 0.05 for both qPCR and immunoblotting analyses.