Figure 1From: Nicotine exposure during differentiation causes inhibition of N-myc expressionnhpESC express nAChR. We analyzed nAChR receptor subunit expression by RT-PCR. Brain and muscle serve as positive controls. Bands were sequenced and demonstrated that the correct gene was amplified. B-actin was used as a control for RNA quality, and water-only reaction was the negative control.Back to article page