Figure 1

GSK-3 inhibition prevents skeletal muscle atrophy induced by pulmonary inflammation. (A) Body weight change of the guinea pigs during the experimental procedures. (B) Effects of repeated LPS exposure and GSK-3 inhibition (SB216763) on skeletal muscle wet weights. (C) The fiber cross-sectional area (CSA) of muscle fibers in the extensor digitorum longus (EDL) muscle of the guinea pigs was determined from laminin-stained cross-sections. Representative laminin-stained (white) cross-sections of the same region within the EDL muscle for each experimental group (20X magnification, scale bar = 50 μm). Histogram of quantitative analysis of the mean Type II cross-sectional area (CSA) (n = 7 per group). (D) Fiber size distribution curves of fiber cross-sectional areas of fibers in the EDL. All data shown represent means ± SEM, n = 9 animals per group. ***p < 0.001 compared with the vc control group; # p < 0.05, ### p < 0.001 refers to a difference between indicated conditions.