Acrolein adduction of proteins involved in NF-κB and JNK signaling. Lung tissue homogenates, collected 6 hrs after a single OVA challenge and/or acrolein exposure, were analyzed for carbonylated proteins by biotin hydrazide labeling and avidin purification. Purified biotinylated proteins were evaluated by Western blot for RelA ( A ), IKKβ ( B ) or JNK ( C ). Representative blots are shown. Band intensities were quantified using Image J and expressed as mean ± SEM (n = 6/group) (*, p < 0.05).