Figure 4
Effect of IGF-1 on protein expression in soft and stiff substrates. (A-B) MLF on tissue culture plate (stiff substrate) were stimulated with IGF-1, TGF-β1, or IGF-1/TGF-β1 for 24 hr. Representative Western blots of αSMA, Col I, and Col III with GAPDH loading control are shown. (C) Densitometry after normalization to loading control. (D) MLF cultured on soft matrix and treated with IGF, TGF-β1, or IGF/ TGF-β1 for 24 hr. Representative Western blots of αSMA with GAPDH loading control are shown. (E) Densitometry after normalization to loading control (n = 3, mean ± SEM).