Figure 6
Co-localization of HIMF with pulmonary vascular smooth muscle. Paraffin-embedded lung sections from normoxic (4 days, 20.8% O2), hypoxic (4 days, 10.0% O2), saline-challenged, and Ova-challenged mice were dual stained with rabbit anti-HIMF polyclonal antibody that was visualized by DyLight 488-conjugated goat anti-rabbit IgG antibody (green; B, F), and mouse anti-α-smooth muscle actin monoclonal antibody that was visualized by DyLight 594-conjugated donkey anti-mouse IgG antibody (red; C, G). Cell nuclei were counterstained with DAPI (blue; A, E). The arrows in the merged images indicate co-localization of HIMF and α-smooth muscle actin (D, H). Scale bar: 50 μm.