Figure 3

Gating strategy for identification respiratory DC subsets. Respiratory dendritic cells (DC) were identified according to CD11c⁺Siglec-F^neg CD49b^neg expression to exclude autofluorescent macrophages and NK cells and further dissected into plasmacytoid DC (pDC) according to 120g8 expression. The pDC negative DC fraction was gated for CD103 to identify CD103⁺ DC. Out of the pDC and CD103+ DC negative fraction, MoDC and CD11b^high DC were separated according to MHC-class-II (I-A/I-E) and CD11b expression. MoDC are CD11b^high and MHC-class-II^med-low whereas CD11b^high DC are CD11b^high and MHC-class-II^high.