Figure 4
From: Cigarette smoke-induced accumulation of lung dendritic cells is interleukin-1α-dependent in mice
Expression of IL-1R1 on non-haematopoetic cells is necessary for smoke-induced expression of CCL20 and GM-CSF . Chimeric mice were generated as follows, WT to WT: C57BL/6 wild type bone marrow transferred into irradiated C57BL/6 wild type mice; KO to KO: IL-1R1-deficient bone marrow transferred into irradiated IL-1R1-deficient mice; KO to WT: IL-1R1-deficient bone marrow transferred into irradiated C57BL/6 wild type mice; WT to KO: C57BL/6 wild type bone marrow transferred into irradiated IL-1R1-deficient mice. Chimeric mice were exposed to cigarette smoke for 4 days. Expression of CCL20 and GM-CSF mRNA was assessed by fluidigm analysis. Results are presented as mean ± SEM of at least five mice per group. Data are representative of at least two independent experiments.