Figure 1

Cigarette smoke-induced dendritic cell accumulation and maturation is IL-1R1-dependent. C57BL/6 wild type, IL-1R1-, and TLR4-deficient mice were exposed to cigarette smoke for 4 days. (A) CD45⁺ whole lung cells were analyzed by flow cytometry for CD11c^hi major histocompatibility class (MHC)-II^hi dendritic cells in room air control wild type, and cigarette smoke-exposed wild type, IL-1R1-, and TLR4-deficient mice. (B) Flow cytometric analysis of CD86⁺ dendritic cells. Shaded gray histogram represents isotype control stain, while the solid line represents CD86-positive staining. (C and D) Quantitative analysis of CD45⁺ CD11c^hi MHC II^hi dendritic cells (left), CD86⁺ dendritic cells (CD11c^hi MHC II^hi) (middle), and mean fluorescence intensity (M.F.I.) of CD86 on dendritic cells (right) of room air and smoke-exposed wild type (WT; white bar), and IL-1R1- or TLR4-deficient (gray bar) mice. Fluidigm analysis of CCL20 mRNA expression in IL-1R1-deficient (E) and TLR4-deficient mice (F), and GM-CSF mRNA expression in TLR4-deficient mice (G). Data presented in A and B are representative of one of five mice from two separate experiments. C to G represent mean ± SEM of at least five mice per group and are representative of at least two independent experiments.