Figure 8
Effect of 25-HC on TLR3 expression in poly(I:C)-treated primary HBEpC. Cells were treated with 10 μM 25-HC or vehicle in the presence of 10 μg/ml poly(I:C). After 24 h, the whole cell lysates were obtained. The expression of TLR3 was evaluated by immunoblotting. Each band intensity was assessed by densitometry. Relative intensity was calculated by dividing the TLR3 band intensity by each appropriate β-actin band intensity. The data are expressed as mean values ± SEM for four separate experiments with HBEpC from two donors. **p < 0.01, compared with the values of vehicle-treated cells. +p < 0.05, compared with the values of each group. N.S. = not significant.