Figure 7
Effect of 25-HC on Interferon regulatory factor 3 (IRF3) and poly(I:C)-induced IFN-β release from HBEpC. (A) Effect of 25-HC on translocation of IRF3 into the nucleus. Cells were treated with 10 μM 25-HC or vehicle. At various time points, the nuclear fraction of the cell lysates was obtained. Translocation of IRF3 into the nucleus was evaluated by immunoblotting. Each band intensity was assessed by densitometry. Relative intensity was calculated by dividing the IRF3 band intensity by each appropriate lamin A/C band intensity. (B) Effect of 25-HC on poly(I:C)-augmented IFN-β release in HBEpC. Cells were treated with 10 μM 25-HC or vehicle in the presence of 10 μg/ml poly(I:C). After 24 h, the supernatants were assayed for IFN-β by ELISA. *p < 0.05, **p < 0.01, compared with the values of control at 0 min. The data are expressed as mean values ± SEM for four separate experiments with HBEpC from two donors. N.D. = not detectable.