Figure 6

Effect of NF-κB inhibitors on 25-HC-augmented IL-8 release in poly(I:C)-treated cells. (A) Effect of a NF-κB inhibitor, CAPE on poly(I:C)-augmented IL-8 release in HBEpC. Cells were treated with various concentrations of CAPE 30 min prior to the treatment with 10 μg/ml poly(I:C). After 24 h, supernatants were harvested and assayed for IL-8. (B-D) Effect of NF-κB inhibitors on 25-HC-augmented IL-8 release in poly(I:C)-treated cells. Various concentrations of a NF-κB inhibitor, CAPE (B), an IκBα inhibitor, BAY 11–7085 (C) or an IKK-2 inhibitor, SC-514 (D) were added before 10 μM 25-HC treatment, and the cells were then cultured in the presence of 10 μg/ml poly(I:C). After 24 h, supernatants were harvested and assayed for IL-8. The data are expressed as mean values ± SEM for three to four separate experiments with HBEpC from two donors. BAY = BAY 11–7085. N.S. = not significant.