Figure 5

Effect of 25-HC on poly(I:C)-augmented IL-8 release from HBEpC. (A) Cells were treated with 10 μM 25-HC or vehicle 15 min prior to the treatment with 10 μg/ml poly(I:C). At various time points after the incubation, supernatants were harvested and assayed for IL-8. (B) Cells were treated with various concentrations of 25-HC or vehicle 15 min prior to the treatment with 10 μg/ml poly(I:C). After 24 h, supernatants were harvested and assayed for IL-8. The data are expressed as mean values ± SEM for three to four separate experiments with HBEpC from two donors. *p < 0.05, **p < 0.01, compared with the values of vehicle-treated cells. +p < 0.05, ++p < 0.01, compared with the values of poly(I:C) or 25-HC-treated control cells.