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Figure 1 | Respiratory Research

Figure 1

From: 25-hydroxycholesterol enhances cytokine release and toll-like receptor 3 response in airway epithelial cells

Figure 1

Effect of oxysterols and liver X receptor ligands on IL-8 release in HBEpC. (A) Cells were treated with 10-5 M of 25-hydroxycholesterol (25-HC). At various time points after the incubation, supernatants were harvested and assayed for IL-8 by ELISA. (B-F) Cells were treated with various concentrations of 25-HC (B), 22-hydroxycholesterol (22-HC) (C), 27-hydroxycholesterol (27-HC) (D), liver X receptor ligands, GW3965 (E), or TO901317 (F). After 24 h, supernatants were harvested and assayed for IL-8. Inserted figures show the effects of hydroxycholesterols and liver X receptor ligands on cell viability. Cells were incubated with various concentrations of 25-HC (B), 22-HC (C), 27-HC (D), GW3965 (E), TO901317 (F) or vehicle for 24 h. Cell viability was assessed by MTT assay. Cell viability is calculated as a percentage of the viable cells in the vehicle treated group. The data are expressed as mean values ± SEM for three to four separate experiments with HBEpC from two donors. *p < 0.05, **p < 0.01; compared with the values of control.

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Respiratory Research

ISSN: 1465-993X

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