Figure 2

The lung epithelial specific Fizz1 overexpression increases the percentage of dendritic cells in the lung. (A) CCSP/Fizz1 mice were fed doxycycline food for 14 days. Total BAL cells increased with Fizz1 overexpression in CCSP/Fizz1 mice. Statistical significance is measured using unpaired student’s t test (n = 11/group). (B) CCSP/Fizz1 mice were fed doxycycline food for 14 days and total lung cells were stained with anti-CD11b and anti-CD45 antibodies. Gating strategy and representation of CD45+ and CD11b + cells in the lungs of Fizz1 overexpressing mice compared to CCSP/Fizz1 mice on no Dox. (C) Fizz1 overexpression has caused increase in the percentage of CD45 and CD11b double positive cells. Statistical significance is measured using unpaired student’s t test (n = 3/group). (D) CCSP/Fizz1 mice were fed doxycycline food for 14 days and total lung cells were stained with anti-CD11c and anti-F4/80 antibodies. Gating strategy and representation of DC, AM and IM in the lungs of Fizz1 overexpressing mice compared to CCSP/Fizz1 mice on no Dox. (E) Mice overexpressing Fizz1 demonstrate increases in the percentage of cells staining for CD 11c that do not stain for F4/80 consistent with changes in dendritic cells. Statistical significance is measured using unpaired student’s t test (n = 3/group). Data represent one of two independent experiments showing similar results.