Figure 1

Supernatants from NTHi slow ciliary beat frequency in a time-and concentration-dependent manner. A) Bovine bronchial epithelial cells (BBECs) were exposed to 0%, 10%, 50% and 100% NTHi supernatant for 10 minutes. Ciliary beat frequency (CBF) was measured each minute. NTHi supernatant exposure rapidly slows CBF at the 50% (*p < 0.05) and 100% (#p < 0.001) concentrations. There is no difference between 10% and media control. (n = 6) B) BBECs were exposed to 50% NTHi supernatant, or 50% NTHi growth medium for up to 48 hours and CBF was measured hourly. The CBF slowing is sustained, with statistically significant CBF slowing at each timepoint compared to control. (*p < 0.05; #p < 0.001 vs. media) (n = 8).