Figure 9
From: Nrf2 protects human alveolar epithelial cells against injury induced by influenza A virus
Nrf2 knockdown sensitizes ATI-like cells to PR8 virus. ATI-like cells were transfected with 100 nmol Nrf2 siRNA or NT siRNA for 24 h as described in the Methods section. Panel I – Nrf2 was knocked down by Nrf2 siRNA in cells harvested at 24 hpi: Lane 1 – control; lane 2 - NT siRNA; lane 3 - Nrf2 siRNA (immunoblotting). Relative expression of these proteins is also shown. Panel II – A higher percentage of necrotic cells was observed in cells transfected with Nrf2 siRNA for 24 h and followed by infection with PR8 virus for 24 h in comparison with cells infected with PR8 virus alone. * Statistically significant increase in percentage of necrotic cells induced by PR8 virus in comparison with control. # Statistically significant increase of ATI-like necrotic cells after transfection with Nrf2 siRNA followed by PR8 in comparison with PR8 virus alone. Data represent results from three independent experiments (Mean ± SEM, * p < 0.05).