Figure 1

Simvastatin inhibits Eotaxin-1 expression in mouse primary tracheal epithelial cells. Mouse primary tracheal epithelial cells grown in air-liquid interface (ALI) were pre-treated with simvastatin (Sim, 10 μM) for 24 hrs, then stimulated with IL-13 (20 ng/mL) and co-incubated with Sim for 48 hrs (total Sim exposure of 72 hrs). Gene expression was evaluated by RT-PCR. Treatment with Sim reduced eotaxin-1 (CCL11) expression by 87.8% (*p < 0.0001 by ANOVA). Simvastatin treatment had no statistically significant effect on basal expression of eotaxin-1 (Con. versus Sim 10 μM; p = NS by ANOVA)