Figure 6

mRNA expression levels of ZO-1 and occludin in lung tissue. RNA was extracted from the lung tissue in both normoxia and hyperoxia groups, converted to cDNA and quantification of ZO-1 and occludin mRNA expression was determined using RT-PCR. β-actin was used as an internal control (A, C). Semiquantitative analysis of ZO-1 and occludin after different days of hyperoxia exposure (B, D). Values are presented as means ± SD; * P < 0.05, ** P < 0.01 versus normoxia group. All experiments were performed independently 4 times.