Figure 4

Monocyte-derived DCs from sarcoidosis patients induce increased TNFα expression. Activation and function of mo-DCs from sarcoidosis patients and healthy controls. (A) After culturing mo-DCs from monocytes, mean expression of DC maturation markers CD40, CD80, CD83 and CD86 was assessed by flowcytometry. (B) Mo-DC induced T cell proliferation was tested in a MLR after 5 days of co-culture with CFSE-labeled naïve CD4⁺ T cells, using flowcytometry. (C, D) Mo-DC induced T-cell differentiation was measured by expression of CD25 (C) and Treg markers (CD25⁺Foxp3⁺CD127 ^low)(D). (E) Cytokine production in DC-T cell co-cultures. Indicated cytokines were measured in the culture supernatants by Luminex. Data are presented as boxplots, whiskers indicate minimum and maximum of the data; * p ≤ 0.05.