Figure 7

Expressions of alveolar epithelial sodium channel(α-ENaC, β-ENaC and γ-ENaC) in alveolar epithelial type II cells pretreated with LY294002(10 μM), Akt inhibitor(100 nM) and SGK1 inhibitor(10 μM) for 30 minutes before insulin treatment for 2 hours were measured by RT-PCR(A) and western blotting(B). Proteins using the same antibodies against α-ENaC, β-ENaC and γ-ENaC plus blocking peptides specific for these antibodies were re-blotted(C). Data are presented as mean ± S.E.M.ΔP < 0.05 vs Control group;*P <# 0.05 vs Insulin group; P < 0.05 vs Insulin + Akt inhibitor group.