Effect of exogenous insulin on the protein expression of α- ENaC(A), β- ENaC(B), and γ-ENaC(C) in rat lung 8 hours after LPS-induced actue lung injury or saline treatment. The number of positive cells was counted in randomly 5 high-power fields of each section and averaged (five sections from 5 rats in each group). The number of cells expressing α-, β-, and γ-ENaC were significantly decreased in LPS-induced actue lung injury, compared with those in positive control group. However, the number of cells expressing α-, β-, and γ-ENaC were strongly increased by insulin treatment, but were decreased by wortmannin. Brown immunostained cell was positive (original magnification 400 ×).ΔP < 0.05 vs Positive control group;*P < 0.05 vs LPS group;# P < 0.05 vs LPS + Insulin group.