Verification of cDC or pDC depletion by flow cytometry. Lung mononuclear cells were stained for DC subset markers. Cells are reported as percent of total population. Macrophages were excluded using FL1 autofluorescence and the macrophage specific marker F4/80. A) Myeloid dendritic cells: B220-, Gr1-, CD11b+ and CD11c+. Wt BALB/c + DT (non-depleted control) vs. DTR Tg BALB/c + DT (depleted) yield a 63% depletion. B) Plasmacytoid dendritic cells: CD11clow, B220+, and PDCA+. Wt + isotype Ab (non-depleted control) vs. Wt Balb/c + mPDCA-1 Ab (depleted) yields a 84% depletion. Data are representative of four to six mice in each group.