Figure 3

Representative images for the dual immunofluorescence detection of CD8⁺GRα⁺ and CD8⁺GRβ⁺ T- cells within human lung tissue from (A&B) a COPD subject and (C&D) a smoking control subject. (A&C) CD8⁺ cells were identified using an Alexa-488 conjugated goat anti-rabbit secondary antibody (Green stain) and GRα⁺ cells were detected using an Alexa-568 conjugated goat anti-mouse secondary antibody (Red stain). (B&D) CD8⁺ cells were identified using an Alexa-568 conjugated goat anti-mouse secondary antibody (Red stain) and GRβ⁺ cells were detected using an Alexa-488 conjugated goat anti-rabbit secondary antibody (Green stain). Cell nuclei were counterstained with 4', 6-diamidino-2-phenylindole (DAPI, blue stain). Magnification × 200. (E&F) Analysis of the number of CD8+ cells that were also positive for GRα and GRβ, within COPD (n = 10) and smoking control (n = 11) follicles. Data presented as mean + SD.