Figure 3
S100A7 is present in the nasal epithelium and seromucous glands. Tissue slides of the human inferior turbinate were stained with (A, B) a monoclonal antibody against S100A7 or (C) an isotype-matched control antibody. Positive staining (brown) was detected in the nasal epithelium and in seromucous glands. Counterstaining with hematoxylin (blue) (magnification 100 ×). (D) Expression of S100A7 mRNA was determined in nasal biopsies (n = 11), primary human nasal epithelial cells (HNEC, n = 6) and FaDu (n = 4) by real-time RT-PCR. Data is expressed in relation to β-actin as 2-ΔCt × 105. (E) Levels of S100A7 in the culture medium from HNEC and FaDu were analyzed by ELISA (n = 4-6). (F) mRNA expression of S100A7 in purified peripheral blood T lymphocytes, B lymphocytes, monocytes, neutrophils and eosinophils using real-time RT-PCR (n = 3-6). Data are presented as mean ± SEM.