Figure 5
From: Oxidative stress induced Interleukin-32 mRNA expression in human bronchial epithelial cells
Knockdown efficiency of c-Jun and CREB mRNA and IL-32 expression induced by IFNγ and H 2 O 2 in HBE cells transfected with c-Jun or CREB siRNAs. c-Jun (A) and CREB (B) mRNA expression levels examined by quantitative real time PCR in HBE cells transfected with indicated siRNAs. Expression levels of c-Jun (A) and CREB (B) by each siRNA transfection were looked by quantitative real time PCR. IL-32 expression was examined by real time PCR in HBE cells transfected with control-siRNA, closed bars, c-Jun-siRNA, open bars, or CREB-siRNA, hatched bars, respectively. Then 48 hours after transfection, cells were stimulated with H2O2 and/or IFNγ, followed by IL-32 quantitative real time PCR of RNA extracted 24 hours after the stimulation (C). The bars represent the means ± SE from 3 different individuals. *p < 0.05 significantly different.