Figure 3

Transcription factors that bind to the IL-32 gene promoter. HBE cells were stimulated with H₂O₂, IFNγ, H₂O₂ + IFNγ, or vehicle for 30 minutes. The ChIP assay was performed to identify which transcription factors bind to the IL-32 gene promoter. Binding activity was compared using quantitative real-time PCR of the IL-32 promoter in DNA from chromatin complexes immunoprecipitated by antibodies to c-Jun (A), CREB (B), and RNA polymerase II (C). The bars show the means ± SE from 3 different individuals. *p < 0.01 significantly different.