Gene expression analysis of a murine model with pulmonary vascular remodeling compared to end-stage IPAH lungs

Respiratory Research

Table 1 Primers used in RT-PCR

Gene Symbol	Assay ID*	RefSeq^†	Context Sequence
Acvrl1	Mm03053695_s1	NM_009612.2	TTTGTGGGAGCACTTGGCCTGTGAC
Bmpr2	Mm01254942_m1	NM_007561.3	AGTATACAGATAGGTGAGTCAACAC
Ccl8	Mm01297184_g1	NM_021443.2	CCATGGAAGCTGTGGTTTTCCAGAC
Ccl9	Mm00441260_m1	NM_011338.2	AGATCACACATGCAACAGAGACAAA
Ear11	Mm00519056_s1	NM_053113.2	CACAACTCCGGCCAGTCATTATTCC
Eng	Mm00468256_m1	NM_001146348.1	AAAAAACACGTGCAGACTCTCCAGT
Gapdh^‡	Mm99999915_g1	NM_008084.2	TGAACGGATTTGGCCGTATTGGGCG
Igj	Mm00461780_m1	NM_152839.2	TCCGAATTGTTGTCCCTTTGAACAA
Mmp12	Mm00500554_m1	NM_008605.3	AAGTTTTCAAGGCACAAACCTCTTC
Mmp13	Mm00439495_g1	NM_008607.1	GAACCACGTGTGGAGTTATGATGAT
Mmp19	Mm00491300_m1	NM_021412.1	TGGTGCTGGGGCCTCGTGGGAAGAC
Nos3	Mm01134921_g1	NM_008713.4	CGGCGTGCTGCGGGATCAGCAACGC
Pdgfa	Mm01205760_m1	NM_008808.3	GGAGGAGGAGACAGATGTGAGGTGA
Vegfa	Mm01281449_m1	NM_001025250.3	ACGTACTTGCAGATGTGACAAGCCA

*Taqman® Gene Expression Assays.
^†NCBI Gene Reference: NCBI transcript ID detected by the assay.
^‡GAPDH: Glyceraldehyde-3-phosphate dehydrogenase was used as an internal control.

ISSN: 1465-993X