Figure 4
Lp-PLA 2 deficiency did not increase airway inflammation and hyperresponsiveness after allergen challenge of passively sensitized Lp-PLA2-/- mice. (A): 9-12 week-old male C57/B6 wild-type and Lp-PLA2-/- mice were sensitized i.v. by different dilutions of serum (results using 1:200 are shown) containing IgE against Aspergillus fumigatus (Af) on day 1, day 2 and day 3, then intranasally challenged with 30 μg Af on day 5. Lung responses were analyzed on day 6. Control mice received naive serum and/or were challenged with PBS. (B): Lung resistance was measured by FlexiVent in response to increasing doses of MCh. (C): BAL was obtained and cytospin preparations of the cell pellet were assessed under light microscope. Differential cell count was performed using total cell counts and Giemsa-stained cytospin preparations. Cells were identified as macrophages (MP), eosinophils (EP), neutrophils (NP) and lymphocytes (LC). (Mean±SEM n=5-11).