Figure 5

Pathway analysis of proteins detected at elevated concentrations during time course analysis in lung following intratracheal instillation of 20 μg carbon nanoparticles (CNP). At day 7 when PMN levels were 5 times higher compared to baseline and a sudden influx of lymphocytes and macrophages was detected, Interleukin-1beta (IL1B), Tumor necrosis factor-alpha (TNF), Factor VII (F7), Interleukin-4 (IL4), Macrophage-derived chemokine (MDC/CCL22) and von Willebrand Factor (vWF) remained at significantly elevated concentrations compared to their baseline levels. The predicted molecular network indicates the role of these markers in airway inflammation, recruitment of lymphocytes and activation of macrophages. Shown are the extracellular space, part of the cell membrane and published interactions are indicated by arrows (line thickness represents the number of references).