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Figure 6 | Respiratory Research

Figure 6

From: Real time analysis of β2-adrenoceptor-mediated signaling kinetics in Human Primary Airway Smooth Muscle Cells reveals both ligand and dose dependent differences

Figure 6

Time taken for a range of concentrations of the β 2 -adrenoceptor agonist isoproterenol (10-8M-10-5M) to initiate an increase in CFP-Epac(dDEP,CD)-VENUS activation. These data were obtained from real time traces comparable to those depicted in Figure 4D. Single hASM cells expressing CFP-Epac(dDEP,CD)-VENUS were excited at 440 nm and the emission ratio (470/535) changes collected in real time. Each data point represents the mean (± SEM) of 4-19 experiments. * denotes P < 0.05.

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