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Figure 4 | Respiratory Research

Figure 4

From: Real time analysis of β2-adrenoceptor-mediated signaling kinetics in Human Primary Airway Smooth Muscle Cells reveals both ligand and dose dependent differences

Figure 4

Time taken for a range of β 2 -adrenoceptor agonists to (A) initiate an increase in CFP-Epac(dDEP,CD)-VENUS activation and (B) to induce a maximal response c.f. initiation. The time between response initiation and maximal response is shown in C. The labelled trace in D depicts how these data were obtained from real time traces. The β2-adrenoceptor agonists studied were isoproterenol (10 μM), salbutamol (1 μM), salmeterol (100 nM), indacaterol (1 μM) and formoterol (1 μM). Single hASM cells expressing CFP-Epac(dDEP,CD)-VENUS were excited at 440 nm and the emission ratio (470/535) changes collected in real time. Each data point represents the mean (± SEM) of 6-19 experiments. * denotes P < 0.05, ***denotes P < 0.001.

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Respiratory Research

ISSN: 1465-993X

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