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Figure 12 | Respiratory Research

Figure 12

From: GDF-15 is abundantly expressed in plexiform lesions in patients with pulmonary arterial hypertension and affects proliferation and apoptosis of pulmonary endothelial cells

Figure 12

Effect of GDF-15 on endothelial cell death. Human pulmonary microvascular endothelial cells (HPMEC) were exposed to hypoxia within an incubator chamber filled with a gas mixture of 0,2% oxygen, 5% carbon dioxide and 94,8% nitrogen placed in a 37°C incubator. Apoptotic cell death was either assessed by measuring the activity of the caspases 3 and 7 (panel A) and by determining the number of Annexin V-positive cells (panel B). Recombinant GDF-15 at a concentration of 5 and 50 ng/ml) reduced hypoxia-induced apoptotic cell death. Data from n = 5 in each group are shown as mean ± SD. * = p < 0.05 compared to control.

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Respiratory Research

ISSN: 1465-993X

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