Figure 1

The immunohistochemical expression of Tn-C (1 A-C) and α-SMA (1 D-F) in bronchial biopsies from study subjects. The figures show representative microphotographs from each category. A: Positivity for Tn-C is seen in tangentially sectioned basal epithelial cells and along the basement membrane (BM) of bronchial epithelium (arrows); scale bar = 0.05 mm. B: Tn-C positivity in basal epithelial cells and in the stroma underneath the BM (arrows); scale bar = 0.05 mm. C: Positivity for Tn-C in basal epithelial cells and in a wide area of stromal connective tissue underneath the BM (arrows); scale bar = 0.05 mm. D: Spindle shaped cells positive for α-SMA (arrows) in a biopsy graded to the category 1-4 positive cells. Smooth muscle of the bronchial wall (SM) or blood vessels (arrow heads) were not counted; scale bar = 0.05 mm. E: A bronchial biopsy graded to the category 5-10 α-SMA positive cells (arrows). Blood vessels (arrow heads) or smooth muscle layer of the bronchial wall (SM) were not counted; scale bar = 0.05 mm. F: More than 10 spindle shaped cells are showing positivity for α-SMA (arrows) in a bronchial biopsy; scale bar = 0.05 mm.