Table 2 Primers used for quantitative PCR analysis of SCGB genes expression
Gene | Accession number | Primers sequence | Annealing temperature (˚C) |
|---|---|---|---|
SCGB3A2 (UGRP1) | NM_054023 | forward,5'-CGGAATTCCCCAGATAACTGTCA-3' | 60 |
reverse, 5'-ACATCTAGACACCAAGTGTGATAGC-3' | |||
SCGB1C1 (RYD5) | NM_145651 | forward, 5'-TGGCCCTCACCCTGTTCTGCATCT-3' | 60 |
reverse, 5'-CACCGTGCTGACTGCCCAGCAGTT-3' | |||
SCGB1D1 (LIPA) | NM_006552 | forward, 5'-CAGTGGTCTGCCAAGCTCTTGG-3' | 60 |
reverse, 5'- CATAGGCCATCGTATCCACGC-3' | |||
SCGB1D2 (LIPB) | NM_006551 | forward, 5'-CCTCTGTTCAAGTTAAGTC-3' | 58 |
reverse, 5'-CCGCAATGAGGCTTCGTTTGG-3' | |||
SCGB1D4 (IIS) | NM_206998 | forward, 5'-CTCACAGCCGAATAAGCCACC-3' | 55 |
reverse, 5'-GTGCAGGGCAAGTGATTTATTAAAGC-3' | |||
SCGB2A1 (MGB2) | NM_002407 | forward, 5'-CTCCTGGAGGACATGGTTG-3' | 60 |
reverse, 5'-CTATGTGACTGGTTGAGG-3' | |||
SCGB2A2 (MGB1) | NM_002411 | forward, 5'-GACAATGCCACTACAAATGCC-3' | 60 |
reverse, 5'-CATTGCTCAGAGTTTCATCCG-3' | |||
SCGB3A1 (UGRP2) | NM_052863 | forward, 5'- CGGAATTCCCCCGCGCCATGAAGCTC-3' | 66 |
reverse, 5'- ACATCTAGAGCCAAACACTGTCAGG-3'. | |||
GAPDH | NM_002046 | forward,5'-GAAGGTGAAGGTCGGAGTC-3' | 60 |
reverse, 5'-GGAAGATGGTGATGGGATT-3' |