Figure 4

Inhibition of AM induced chemotactic activity from tiotropium on neutrophils is predominantly driven by M3R-blockage (n = 10): Tiotropium 30 nM, 4-DAMP 100 nM (each: p < 0.01 vs. LPS stimulation alone), Ipratropium 30 nM, Gallamine 100 μM, Tubocurarine 100 μM, Telenzepine 10 nM. Doses were adapted from earlier studies and customized to our assay conditions [43, 63, 64].