Figure 6
Effect of CXCR4 shRNA delivery on bone marrow cell migration to rat lung: (A) GFP expression. Proteins were isolated form rat lungs and Western blot was performed for analysis of GFP protein expression. Quantitative data (upper panel), setting hypoxia BMC as 1, and representative images (lower panel). *p < 0.05 as compared with other groups. (B) c-kit expression. Proteins were isolated form rat pulmonary artery and Western blot was performed for analysis of c-kit expression. Quantitative data (upper panel), setting normoxia control as 1, and representative images (lower panel). *p < 0.05 as compared with other hypoxia groups. # p > 0.05 as compared with normoxia control. n = 3 for each groups. BMC = transplantation of bone marrow cells without shRNA, BMC+S-RNA = transplantation of bone marrow cells with scrambled shRNA, BMC+ CXCR shRNA = transplantation of bone marrow cells with CXCR4 shRNA.