Figure 3

Diacylglycerol kinase inhibitor R59949 diminished HPV specifically. The effect of the diacylglycerol kinase inhibitor R59949 as well as its solvent on the normoxic vascular tone, the strength of HPV, and the strength of U46619-induced vasoconstriction was investigated in isolated wild-type mouse lungs. (A) Increase in normoxic pulmonary arterial pressure (ΔPAP), assessed directly before each hypoxic ventilation maneuver. (B) Strength of HPV referenced to the effect of the second hypoxic ventilation maneuver (= 100%). Absolute values of HPV for the second hypoxia maneuver prior to R59949 application were 0.7 ± 0.1 (n = 5). (C) Strength of the U46619-induced vasoconstriction, referenced to the effect of the second U46619 application (= 100%). Data are derived from n = 5 isolated lung preparations each. * Significant differences compared to control experiments with application of the solvent only (p < 0.05).