The actions of PlGF on endothelial cell wound healing. (A) Human pulmonary microvascular endothelial cell scratch assay in the presence of vehicle (panel 1), PlGF (40 ng/ml; panel 2) and PlGF (160 ng/ml; panel 3). (B) Mean (±SEM) percentage wound closure over a 24-hour period in the presence of PlGF under normoxic conditions. (C) Mean (±SEM) percentage wound closure over a 24-hour period in the presence of VEGFA (8 ng/ml)-PlGF (40 ng/ml) and VEGFA (8 ng/ml)-PlGF (160 ng/ml) in normoxic human pulmonary microvascular endothelial cells. * signifies a significant increase in wound healing compared to vehicle, † signifies significant difference from VEGFA alone (8 ng/ml) (P < 0.05, ANOVA, post hoc Student Newman Keuls). N = 6-9 per group.