Figure 7

CSC induces cytokine production from CD8⁺ T cells. CD8⁺ T cells from COPD patients (n = 10) were treated with CSC (10 μg/ml or 50 μg/ml), DMSO, or medium for 24 hours. The supernatants were collected and cytokine expression was examined using the Human ProInflammatory 7-Plex Tissue Culture Plate from Meso Scale Discovery. There was no significant change in cytokine production between the untreated cells or those which were treated with DMSO. At 50 μg/ml of CSC treatment, there was a significant increase in cytokine expression for IL-1β (A), IL-10 (D), IL-12p70 (E), TNFα (F), and IFNγ (G) compared to DMSO. There was no change in IL-8 (C) expression. There was also a significant increase in cytokine production at 50 μg/ml compared to cells treated with 10 μg/ml of CSC for IL-1β (A), IL-6 (B) IL-10 (D), IL-12p70 (E), TNFα (F), and IFNγ (G). Data are represented as mean ± SEM. * indicates p-values < 0.05; ** indicates p-values < 0.01 and *** indicates p-values < 0.0001. nd = not determined.