Figure 4

Microinjection of EV71 to nucleus tractus solitarii resulted in the accumulation of viral antigens in both nucleus tractus solitarii and ventral medulla with neuronal loss in ventral medulla. EV71/MP4 (3 × 10⁷ PFU/ml) was bilaterally microinjected into the nucleus tractus solitarii (NTS) of 8-week-old ICR mice in a volume of 0.2 μl. Sagittal cryosections of brain tissues were stained with Nissl strain or anti-EV71 or anti-NeuN antibody (A). Quantification of EV71-positive cells in ventral medulla (VM) (B) and different brain regions (C), and NeuN-positive cells in VM (D) and NTS (E). CB: cerebellum; black square: remote area; red square: VM area; blue square: NTS. Data represent means ± S.E.M. of 3 mice with 3 sections per mouse. *, P < 0.001.