Figure 1
IP-10 significantly enhances the adhesiveness of eosinophils. (A) IP-10 significantly augments eosinophil adhesion to rh-ICAM-1-coated plates. Eosinophils (100 μl of 1 × 105 cells/ml in HBSS/gel) obtained from different healthy donors, were incubated with IP-10 (10-100 nM) at 37°C for 20 min in rh-ICAM-1-coated plates. The adhesiveness of the eosinophils was then assessed by assay of residual EPO activity. Data are shown as means ± SEM of 5 experiments using cells from different donors. (B) IP-10 does not modify eosinophil adhesion to rh-VCAM-1-coated plates. Eosinophils were incubated with IP-10 (10-100 nM) in rh-VCAM-1-coated plates, and the adhesiveness of the eosinophils was examined. Data are shown as means ± SEM of 5 experiments using cells from different donors. (C) Time course of IP-10 induction of eosinophil adhesion to ICAM-1 coated plates. Eosinophils were incubated with or without IP-10 (100 nM) for periods ranging from 5 to 60 min. The kinetics of eosinophil adhesion to ICAM-1 coated plates was determined by aspiration of non-adhered cells at the indicated times, followed by washing every 5 min for the next 10 min and then every 10 min over the next 50 min, following which adhesion was assayed. Data are shown as means ± SEM of 6 experiments using cells from different donors. Open and filled bars, eosinophil adhesion without and with IP-10, respectively. *P < 0.05 versus without IP-10.