Figure 6
From: Pleiotropic effects of statins in distal human pulmonary artery smooth muscle cells
Effect of statins on TGF-β1-stimulated (10 ng/ml) ET-1 release from human PASMCs. (A) Release of ET-1 in conditioned medium was attenuated in a concentration-dependent manner by lipophilic statins, but not by pravastatin, with simvastatin being more potent than atorvastatin. (B) Effect of simvastatin reversed by co-treatment with mevalonate (MVA, 100 μM) or geranylgeranylpyrophosphate (GGPP; 10 μM), but not by farnesylpyrophosphate (FPP; 10 μM). (C) Inhibitory effect of fluvastatin and NO-releasing derivatives of pravastatin (6550) and fluvastatin (6553) also reversed by MVA. Data are mean ± SEM of replicate values (n = 4) from representative IPAH cell isolates and expressed either as the amount of ET-1 detected (per 106 cells) in conditioned medium (A) or as a proportion of that measured in control cells treated with TGF-β1 alone (B-C). SF = serum free untreated cells.