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Figure 3 | Respiratory Research

Figure 3

From: Conditional deletion of epithelial IKKβ impairs alveolar formation through apoptosis and decreased VEGF expression during early mouse lung morphogenesis

Figure 3

Generation of Nkx2.1/IKKβF/F double transgenic mice. (A) Simplified breeding schema representing cross of Nkx2.1-Cre and homozygous IKKβF/F mice resulting in deletion of exon 3 of IKKβ gene. (B) Genotyping PCR analysis identifying double transgenic (Nkx2.1/IKKβF/F) and control (IKKβF/F) progeny. (C) PCR primer design to confirm lung epithelium selective deletion of IKKβ. (D) Real-time quantitative PCR and (E) Immunohistochemistry confirming decreased expression of IKKβ in lung tissue. [Data are expressed as Mean ± SEM, *p < 0.05 in D; Arrows pointing to IKKβ-positive alveolar type II cells in top panel and IKKβ-negative alveolar type II cells in bottom panel in (E)].

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Respiratory Research

ISSN: 1465-993X

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